WB
Various lysates were subjected to SDS PAGE followed by western blot with HGF alpha antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HGF alpha antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGF Alpha antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | HGF Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Recombinant human HGF protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.35mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HGF |
|---|---|
| Gene Synonyms | SF HGFB HPTA F-TCF DFNB39 |
| Gene Full Name | hepatocyte growth factor |
| Gene Summary | This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015] |
| Alternative Names | HGF Alpha Hepatocyte growth factor Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain Hepatopoietin-A |
| Molecular Weight(MW) | 69kDa |
| Cellular Localization | extracellular region, extracellular space, membrane, platelet alpha granule lumen. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with HGF alpha antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HGF alpha antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGF Alpha antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
