Application
Titration ELISA of anti-HIV-1 p24 antibody PM-6585 with 100 ng of recombinant HIV-1 p24 protein.Application
Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).Application
Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.| Product Name | HIV-1 p24 Antibody [7F4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3).1) Goto T, Nakai M, and Ikuta K. The life-cycle of human immunodeficiency virus type 1. Micron 1998; 29:123-38. |
| Immunogen | Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| PM-6585 can be used for detection of p24 by Western blot or ELISA at 0.2 – 0.5μg/mL. | |
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GAG |
|---|---|
| Alternative Names | HIV-1 p24 Antibody [7F4] : |
| Molecular Weight(MW) | Observed: 24 kDa, 41 kDa, and 55 kDa |
| Tissue Specificity | By Western blot, anti-HIV-1 p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 p24 and to its precursors p41 and p55, respectively, in HIV-1 samples. |
Application
Titration ELISA of anti-HIV-1 p24 antibody PM-6585 with 100 ng of recombinant HIV-1 p24 protein.
Application
Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).
Application
Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.| Positive Control | 1) Cat. No. 95-153 - Recombinant HIV-1 p24 |
|---|---|
| Application Notes | PM-6585 can be used for detection of p24 by Western blot or ELISA at 0.2 – 0.5μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | HIV-1 p24 Monoclonal Antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
