Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody.Application
Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | hnRPD (phospho Ser83) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ROD, AUF1, HNRPD |
| Product description | Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.1) Kajita Y., J. Biol. Chem. 270:22167-22175(1995). |
| Immunogen | hnRPD (phospho Ser83) antibody was raised against a peptide sequence around phosphorylation site of Serine 83 (N-S-S (p)-P-R) derived from Human hnRPD . |
| Modification | p-Ser83 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRNPD |
|---|---|
| Alternative Names | ROD AUF1 HNRPD |
| Molecular Weight(MW) | 38 kDa |
| Tissue Specificity | The antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83. |
| Entrez Gene | 3184 |
|---|---|
| Protein Accession | NP_112738.1 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody.
Application
Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
