Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Product Name | HSF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock factor protein 4, HSF 4, hHSF4, Heat shock transcription factor 4, HSTF 4, HSF4 |
| Product description | Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This HSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the N-terminal region of human HSF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSF4 |
|---|---|
| Alternative Names | Heat shock factor protein 4 HSF 4 hHSF4 Heat shock transcription factor 4 HSTF 4 HSF4 |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
