Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in A431,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).| Product Name | HSPD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60 |
| Product description | HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.1) Venner T.J., Singh B., Gupta R.S.DNA Cell Biol. 9:545-552(1990) |
| Immunogen | This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the C-terminal region of human HSPD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | HSPD1 |
|---|---|
| Alternative Names | 60 kDa heat shock protein mitochondrial 60 kDa chaperonin Chaperonin 60 CPN60 Heat shock protein 60 HSP-60 Hsp60 HuCHA60 Mitochondrial matrix protein P1 P60 lymphocyte protein HSPD1 HSP60 |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in A431,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
