Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.Application
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.| Product Name | IDH3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDH3A, |
| Product description | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD (+)-dependent isocitrate dehydrogenase.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD (+)-dependent isocitrate dehydrogenase.1) Soundar, S., (2003) J. Biol. Chem. 278 (52), 52146-52153. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IDH3A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| IDH3A antibody can be used for detection of IDH3A by ELISA at 1:312500. IDH3A antibody can be used for detection of IDH3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IDH3A |
|---|---|
| Alternative Names | IDH3A |
| Molecular Weight(MW) | 40 kDa |
Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Application
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | IDH3A antibody can be used for detection of IDH3A by ELISA at 1:312500. IDH3A antibody can be used for detection of IDH3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IDH3A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
