Application
Immunofluorescence staining of methanol-fixed HeLa cells using IKK-β (Ab-199).Application
Western blot analysis of lysed extracts from MDA cells using IKK-β (Ab-199).| Product Name | IKK beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKK2, IKKB, IMD15, NFKBIKB, IKK-beta, I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK2, IkBKB |
| Product description | Acts as part of the IKK complex in the conventional pathway of NF-κ-B activation and phosphorylates inhibitors of NF-κ-B thus leading to the dissociation of the inhibitor/NF-κ-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.1) Chen RA, et al. PLoS Pathog. 2008 Feb 8;4 (2): e22Yen CJ, et al. Cancer Res. 2008 Apr 15;68 (8): 2632-40 |
| Immunogen | IKK-beta (Ab-199) antibody was raised against a peptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-β. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKB |
|---|---|
| Alternative Names | IKK2 IKKB IMD15 NFKBIKB IKK-beta I-kappa-B kinase 2 I-kappa-B-kinase beta IKK2 IkBKB |
| Molecular Weight(MW) | 87 kDa |
| Tissue Specificity | This antibody detects endogenous level of total IKK-βprotein. |
| Entrez Gene | 3551 |
|---|---|
| Protein Accession | NP_001547.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using IKK-β (Ab-199).
Application
Western blot analysis of lysed extracts from MDA cells using IKK-β (Ab-199).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
