Application
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 μg/mL.Application
Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 μg/mL.Application
Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.| Product Name | IKK beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKK beta Antibody: IKK2, IKKB, IMD15, NFKBIKB, IKK-beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, I-kappa-B-kinase beta |
| Product description | IKK beta Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for NF-κB activation. IKKβ phosphorylates both IκB-alpha and IκB-beta. IKKβ is expressed in variety of human tissues.1) DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 1997;388:548-54 |
| Immunogen | IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid. The immunogen is located within amino |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKB |
|---|---|
| Alternative Names | IKK beta Antibody: IKK2 IKKB IMD15 NFKBIKB IKK-beta Inhibitor of nuclear factor kappa-B kinase subunit beta I-kappa-B kinase 2 I-kappa-B-kinase beta |
| Molecular Weight(MW) | 87 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | This polyclonal antibody has no cross response to IKKa or IKKy. |
Application
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 μg/mL.
Application
Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 μg/mL.
Application
Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IKK beta antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IKK beta Antibody is supplied in PBS containing 0.02% sodium azide. |
