Application
Western blot analysis of IKK epsilon in Jurkat whole cell lysate with IKK epsilon/IKK-i antibody at 1 μg/mL.Application
Immunohistochemistry of IKK epsilon in human pancreas tissue with IKK epsilon antibody at 10 μg/mL.| Product Name | IKK epsilon Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon |
| Product description | IKK epsilon Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.1) Peters RT, Liao SM, Maniatis T. IKK epsilonpsilon is part of a novel PMA-inducible IκB kinase complex. Mol Cell 2000;5(3):513-22 |
| Immunogen | IKK epsilon antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IKK epsilon antibody can be used for detection of IKK epsilon by Western blot at 1 μg/mL. An 80 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKE |
|---|---|
| Alternative Names | IKK epsilon Antibody: IKKE IKKI IKK-E IKK-i IKKE KIAA0151 Inhibitor of nuclear factor kappa-B kinase subunit epsilon Inducible I kappa-B kinase I-kappa-B kinase epsilon |
| Molecular Weight(MW) | 80 kDa |
| Sequence Similarities | 3) Cat. No. 11-101 - Human Pancreas Tissue Slide |
| Tissue Specificity | It has no cross response to IKKa, IKKb, or IKKy. |
Application
Western blot analysis of IKK epsilon in Jurkat whole cell lysate with IKK epsilon/IKK-i antibody at 1 μg/mL.
Application
Immunohistochemistry of IKK epsilon in human pancreas tissue with IKK epsilon antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | IKK epsilon antibody can be used for detection of IKK epsilon by Western blot at 1 μg/mL. An 80 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IKK epsilon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide. |
