Application
To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-16.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.| Product Name | IL-10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A |
| Immunogen | E.coli derived Recombinant Rat IL-10 |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 8.0-16.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Rat IL-10 when used with ProSci’s Polyclonal Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL10 |
|---|---|
| Alternative Names | Interleukin 10Interleukin-10 Cytokine synthesis inhibitory factor IL-10 CSIF TGIF GVHDS IL10A |
| Entrez Gene | 3586 |
|---|---|
| Protein Accession | NP_000563.1 |
Application
To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-16.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.| Application Notes | ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 8.0-16.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Rat IL-10 when used with ProSci’s Polyclonal Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
