Application
Western blot analysis using IL - 10 monoclonal antibody against truncated IL - 10 recombinant protein.| Product Name | IL-10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A |
| Product description | Interleukine 10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down- regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.1) Vieira P, et al. PNAS, 1991.88:1172-1176. |
| Immunogen | Ni-NTA purified recombinant human IL-10 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL10 |
|---|---|
| Alternative Names | Cytokine synthesis inhibitory factor IL-10 CSIF TGIF GVHDS IL10A |
Application
Western blot analysis using IL - 10 monoclonal antibody against truncated IL - 10 recombinant protein.| Application Notes | Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | to IL-10 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
