Application
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 ug/ml overnight at 4˚C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.Application
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.Application
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 8.0-9.0 ug/ml of this antibody will detect recombinant human IL-11 when used with ProSci's biotinylated antigen affinity purified anti-human IL-11 (XP-5164Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.| Product Name | IL-11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin 11Interleukin-11, Adipogenesis inhibitory factor, IL-11, AGIF |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human IL-11 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL), a concentration of 2.4 - 3.6 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11(XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL. Western blot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL11 |
|---|---|
| Alternative Names | Interleukin 11Interleukin-11 Adipogenesis inhibitory factor IL-11 AGIF |
Application
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 ug/ml overnight at 4˚C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
Application
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Application
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 8.0-9.0 ug/ml of this antibody will detect recombinant human IL-11 when used with ProSci's biotinylated antigen affinity purified anti-human IL-11 (XP-5164Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL), a concentration of 2.4 - 3.6 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11(XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL. Western blot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-11 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
