Application
Immunohistochemistry of IL-31 in rat skeletal muscle tissue with IL-31 antibody at 10 μg/mL.Application
Western blot analysis of IL-31 in RAW264.7 cell lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.| Product Name | IL-31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-31 Antibody: 1700013B14Rik, Interleukin-31, IL-31 |
| Product description | IL-31 Antibody: Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.1) Dillon SR, Sprecher C, Hammond A, et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat. Immunol. 2004; 5:752-60. |
| Immunogen | IL-31 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of mouse IL-31. The immunogen is located within amino acids 30 - 80 of IL-31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IL-31 antibody can be used for the detection of IL-31 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Il31 |
|---|---|
| Alternative Names | IL-31 Antibody: 1700013B14Rik Interleukin-31 IL-31 |
| Sequence Similarities | 2) Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate |
Application
Immunohistochemistry of IL-31 in rat skeletal muscle tissue with IL-31 antibody at 10 μg/mL.
Application
Western blot analysis of IL-31 in RAW264.7 cell lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | IL-31 antibody can be used for the detection of IL-31 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-31 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-31 Antibody is supplied in PBS containing 0.02% sodium azide. |
