Application
Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 μg/mL.Application
Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.| Product Name | IL-31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-31 Antibody: 1700013B14Rik, Interleukin-31, IL-31 |
| Product description | IL-31 Antibody: Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.1) Dillon SR, Sprecher C, Hammond A, et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat. Immunol. 2004; 5:752-60. |
| Immunogen | IL-31 antibody was raised against a 18 amino acid synthetic peptide from near the center of human IL-31. The immunogen is located within amino acids 60 - 110 of IL-31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| IL-31 antibody can be used for the detection of IL-31 by Western blot at 2.5 - 10 μg/mL. Despite its predicted size, IL-31 runs at approximately 27 - 30 kDa in SDS-PAGE. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Il31 |
|---|---|
| Alternative Names | IL-31 Antibody: 1700013B14Rik Interleukin-31 IL-31 |
Application
Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 μg/mL.
Application
Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | IL-31 antibody can be used for the detection of IL-31 by Western blot at 2.5 - 10 μg/mL. Despite its predicted size, IL-31 runs at approximately 27 - 30 kDa in SDS-PAGE. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-31 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-31 Antibody is supplied in PBS containing 0.02% sodium azide. |
