Application
Western blot analysis of IL-37 in human lung tissue lysate with IL-37 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.Application
Immunofluorescence of IL-12A in human kidney tissue with IL-12A antibody at 20 μg/mL.Application
Immunohistochemistry of IL-12A in human kidney tissue with IL-12A antibody at 5 μg/mL.| Product Name | IL-37 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-37 Antibody : FIL1, FIL1Z, IL-1H, IL-37, IL1F7, IL1H4, IL-1F7, IL-1H4, IL1RP1, IL-1RP1, FIL1(ZETA), Interleukin-37, FIL1 zeta |
| Product description | Interleukin 37 (IL-37) is a member of the interleukin 1 cytokine family (reviewed in 1). It can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). IL-37 also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18 (1). IL-37 can also interact with Smad3 and expression of IL-37 in macrophages or epithelial cells resulted in near complete suppression of pro-inflammatory cytokines, suggesting that IL-37 may act as a natural suppressor of the innate inflammatory and immune responses (2,3).1) Boraschi D, Lucchesi D, Hainzl S, et al. IL-37: a new anti-inflammatory cytokine of the IL-1 family. Eur. Cytokine Netw. 2011; 22:127-47. |
| Immunogen | IL-37 antibody was raised against a 17 amino acid peptide near the amino terminus of human IL-37. The immunogen is located within amino acids 30 - 80 of IL-37 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IL-37 antibody can be used for detection of IL-37 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL37 |
|---|---|
| Alternative Names | IL-37 Antibody : FIL1 FIL1Z IL-1H IL-37 IL1F7 IL1H4 IL-1F7 IL-1H4 IL1RP1 IL-1RP1 FIL1(ZETA) Interleukin-37 FIL1 zeta |
| Molecular Weight(MW) | Predicted: 24 kDa Observed: 23kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | IL-37 antibody is human specific. Multiple isoforms of IL-37 are known to exist; this antibody will only detect the largest isoform. IL-37 antibody is predicted to not cross-react with IL-36. |
Application
Western blot analysis of IL-37 in human lung tissue lysate with IL-37 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.
Application
Immunofluorescence of IL-12A in human kidney tissue with IL-12A antibody at 20 μg/mL.
Application
Immunohistochemistry of IL-12A in human kidney tissue with IL-12A antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | IL-37 antibody can be used for detection of IL-37 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-37 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IL-37 Antibody is supplied in PBS containing 0.02% sodium azide. |
