| Product Name | IL-7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin 7Interleukin-7IL-7, IL-7 |
| Product description | Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4 and IL-6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. |
| Immunogen | The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-7 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL7 |
|---|---|
| Alternative Names | Interleukin 7Interleukin-7IL-7 IL-7 |
| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-7 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
