Application
Western blot analysis of ILK using rabbit polyclonal ILK Antibody (pS246) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.Application
Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ILK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2 |
| Product description | Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.1) Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003). |
| Immunogen | This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ILK |
|---|---|
| Alternative Names | Integrin-linked protein kinase 59 kDa serine/threonine-protein kinase ILK-1 ILK-2 p59ILK ILK ILK1 ILK2 |
| Molecular Weight(MW) | 51 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of ILK using rabbit polyclonal ILK Antibody (pS246) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.
Application
Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
