Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Confocal immunofluorescent analysis of IMPDH1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
IMPDH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | IMPDH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inosine-5'-monophosphate dehydrogenase 1 {ECO:0000255|HAMAP-Rule:MF_03156}, IMP dehydrogenase 1 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPD 1 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPDH 1 {ECO:0000255|HAMAP-Rule:MF_03156}, 111205 {ECO:0000255|HAMAP-Rule:MF_03156} |
| Product description | The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene.1) Ohmann, E.L., et al. Pediatr Transplant 14(7):891-895(2010) Gensburger, O., et al. Pharmacogenet. Genomics 20(9):537-543(2010) Kagaya, H., et al. Basic Clin. Pharmacol. Toxicol. 107(2):631-636(2010) Ohmann, E.L., et al. J. Heart Lung Transplant. 29(5):509-516(2010) Shumei, L., et al. Adv. Exp. Med. Biol. 664, 293-297 (2010) : |
| Immunogen | This IMPDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-514 amino acids from the C-terminal region of human IMPDH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IMPDH1 |
|---|---|
| Alternative Names | Inosine-5'-monophosphate dehydrogenase 1 {ECO:0000255|HAMAP-Rule:MF_03156} IMP dehydrogenase 1 {ECO:0000255|HAMAP-Rule:MF_03156} IMPD 1 {ECO:0000255|HAMAP-Rule:MF_03156} IMPDH 1 {ECO:0000255|HAMAP-Rule:MF_03156} 111205 {ECO:0000255|HAMAP-Rule:MF_03156} |
| Molecular Weight(MW) | 55 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Confocal immunofluorescent analysis of IMPDH1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
IMPDH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
