Application
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IP-10 (XP-5203Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.| Product Name | IP-10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C7, IFI10, INP10, IP-10, crg-2, mob-1, SCYB10, gIP-10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10 |
| Product description | Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIP-10 (human IP-10). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIP-10 (100.0 ng/mL), a concentration of 2.0 - 3.0 μg/mL of this antibody is required. ELISA: To detect hIP-10 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10. Sandwich: To detect hIP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IP-10 (XP-5203Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10. Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL10 |
|---|---|
| Alternative Names | C7 IFI10 INP10 IP-10 crg-2 mob-1 SCYB10 gIP-10 C-X-C motif chemokine 10 10 kDa interferon gamma-induced protein Gamma-IP10 |
Application
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IP-10 (XP-5203Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIP-10 (100.0 ng/mL), a concentration of 2.0 - 3.0 μg/mL of this antibody is required. ELISA: To detect hIP-10 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10. Sandwich: To detect hIP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IP-10 (XP-5203Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10. Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IP-10 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
