Application
Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 μg/mL.Application
Immunofluorescence of IRAK in HeLa cells with IRAK antibody at 20 μg/mL.Application
Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody 1 μg/mL.| Product Name | IRAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).1) Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996;271:1128-31. |
| Immunogen | IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIPWB |
| IRAK antibody can be used for Western blot at 1 μg/mL and for immunoprecipitation with 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRAK1 |
|---|---|
| Alternative Names | IRAK Antibody: IRAK pelle IRAK Interleukin-1 receptor-associated kinase 1 IRAK-1 |
| Molecular Weight(MW) | Predicted: 78 kDa Observed: 80 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family. |
Application
Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 μg/mL.
Application
Immunofluorescence of IRAK in HeLa cells with IRAK antibody at 20 μg/mL.
Application
Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IRAK antibody can be used for Western blot at 1 μg/mL and for immunoprecipitation with 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRAK Antibody is supplied in PBS containing 0.02% sodium azide. |
