Application
Immunohistochemistry of IRAK in rat liver tissue with IRAK antibody at 2.5 μg/mL.Application
Western blot analysis of IRAK in HeLa lysates with IRAK antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IRAK Antibody [1F12C1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).1) Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31. |
| Immunogen | Recombinant protein corresponding to amino acids 618 - 712 of human IRAK. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| RAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRAK1 |
|---|---|
| Alternative Names | IRAK Antibody [1F12C1] : IRAK pelle |
Application
Immunohistochemistry of IRAK in rat liver tissue with IRAK antibody at 2.5 μg/mL.
Application
Western blot analysis of IRAK in HeLa lysates with IRAK antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 95-112 - IRAK Recombinant Protein |
|---|---|
| Application Notes | RAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
