Application
Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 μg/mL.Application
Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 μg/mL.Application
Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 μg/mL.| Product Name | IRAK-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.1) Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5. |
| Immunogen | IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2. The immunogen is located within amino acids 530 - 580 of IRAK-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRAK2 |
|---|---|
| Alternative Names | IRAK-2 Antibody: IRAK-2 Interleukin-1 receptor-associated kinase-like 2 IRAK-2 |
| Molecular Weight(MW) | 65 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Anti-IRAK2 has no cross response to IRAK. |
Application
Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 μg/mL.
Application
Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 μg/mL.
Application
Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
