Application
Immunofluorescence of IRAK2 in HeLa cells with IRAK antibody at 10 μg/mL.Application
Western blot analysis of IRAK2 in K562 whole cell lysate with IRAK2 antibody at 1:500 dilution.| Product Name | IRAK-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.1) Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996;271:1128-31. |
| Immunogen | IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2. The immunogen is located within amino acids 560 - 610 of IRAK-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| IRAK-2 antibody can be used for detection of IRAK2 by Western blot 0.5 μg/mL. A 65 kDa band can be detected. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRAK2 |
|---|---|
| Alternative Names | IRAK-2 Antibody: IRAK-2 Interleukin-1 receptor-associated kinase-like 2 IRAK-2 |
| Molecular Weight(MW) | 65 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Anti-IRAK2 has no cross response to IRAK. |
Application
Immunofluorescence of IRAK2 in HeLa cells with IRAK antibody at 10 μg/mL.
Application
Western blot analysis of IRAK2 in K562 whole cell lysate with IRAK2 antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | IRAK-2 antibody can be used for detection of IRAK2 by Western blot 0.5 μg/mL. A 65 kDa band can be detected. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
