Application
Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 μg/mL.Application
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 μg/mL.Application
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | IRAK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.1) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5. |
| Immunogen | IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a. The immunogen is located within the last 50 amino acids of IRAK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Irak2 |
|---|---|
| Alternative Names | IRAK2 Antibody: IRAK-2 AI649099 6330415L08Rik Interleukin-1 receptor-associated kinase-like 2 IRAK-2 |
| Molecular Weight(MW) | Predicted: 68 kDa Observed: 65 kDa |
| Sequence Similarities | 3) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK. |
Application
Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 μg/mL.
Application
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 μg/mL.
Application
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
