Application
Western blot analysis of IRAK2 in A-20 whole cell lysate with IRAK2 antibody (C2) at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | IRAK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.1) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5. |
| Immunogen | RAK2 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mouse IRAK2a which is common to all four isoforms. The immunogen is located within the last 50 amino acids of IRAK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IRAK2 antibody can be used for detection of mouse IRAK2 by Western blot at 0.5 - 2 μg/mL. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Irak2 |
|---|---|
| Alternative Names | IRAK2 Antibody: IRAK-2 AI649099 6330415L08Rik Interleukin-1 receptor-associated kinase-like 2 IRAK-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | Anti-IRAK2 has no cross response to IRAK. |
Application
Western blot analysis of IRAK2 in A-20 whole cell lysate with IRAK2 antibody (C2) at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | IRAK2 antibody can be used for detection of mouse IRAK2 by Western blot at 0.5 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
