Application
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 μg/mL.Application
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 μg/mL.Application
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 μg/mL..| Product Name | IRF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRF3 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.1) Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54. |
| Immunogen | IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3. The immunogen is located within amino acids 150 - 200 of IRF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRF3 antibody can be used for detection of IRF3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL. | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF3 |
|---|---|
| Alternative Names | IRF3 Antibody: Interferon regulatory factor 3 IRF-3 |
| Molecular Weight(MW) | Predicted: 50 kDa Observed: 50 kDa |
| Sequence Similarities | 3) Cat. No. 1225 - Ramos Cell Lysate |
Application
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 μg/mL.
Application
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 μg/mL.
Application
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 μg/mL..| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | IRF3 antibody can be used for detection of IRF3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRF3 Antibody is supplied in PBS containing 0.02% sodium azide. |
