Application
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 μg/mL.Application
Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.| Product Name | IRF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRF8 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases, but IRF8 mediates activation of NF-κB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.1) Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54 |
| Immunogen | IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRF8. The immunogen is located within the last 50 amino acids of IRF8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| IRF8 antibody can be used for detection of IRF8 by Western blot at 1 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF3 |
|---|---|
| Alternative Names | IRF8 Antibody: Interferon regulatory factor 3 IRF-3 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 μg/mL.
Application
Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.| Positive Control | 1) Cat. No. 1314 - Human Thymus Tissue Lysate |
|---|---|
| Application Notes | IRF8 antibody can be used for detection of IRF8 by Western blot at 1 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRF8 Antibody is supplied in PBS containing 0.02% sodium azide. |
