Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Ab-636). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using IRS-1 (Ab-636).Application
Western blot analysis of lysed extracts from 293 cells using IRS-1 (Ab-636).| Product Name | IRS 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit1) Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1): 63-76Kadowaki T, et al. (2000) J Clin Invest; 106 (4): 459-465Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97 (5): 2355-2360 |
| Immunogen | IRS-1 (Ab-636) antibody was raised against a peptide sequence around aa.634~638 (P-M-S-P-K) derived from Human IRS-1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRS1 |
|---|---|
| Alternative Names | HIRS-1 BHLHB26 IRS-1 IRS1 |
| Molecular Weight(MW) | 180 kDa |
| Tissue Specificity | This antibody detects endogenous level of total IRS-1 protein. |
| Entrez Gene | 3667 |
|---|---|
| Protein Accession | NP_005535.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Ab-636). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using IRS-1 (Ab-636).
Application
Western blot analysis of lysed extracts from 293 cells using IRS-1 (Ab-636).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
