Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Phospho-Ser639).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using IRS-1 (Phospho-Ser639).| Product Name | IRS 1 (phospho Ser639) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit1) Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1): 63-76Steppan CM, et al. (2005) Mol Cell Biol; 25 (4): 1569-1575Batty IH, et al. (2004) Biochem J; 379 (Pt 3): 641-651 |
| Immunogen | IRS-1 (Phospho-Ser639) antibody was raised against a peptide sequence around phosphorylation site of serine 639 (P-K-S (p) -V-S) derived from Human IRS-1. |
| Modification | p-Ser639 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRS1 |
|---|---|
| Alternative Names | HIRS-1 NFKB3 IRS-1 IRS1 |
| Molecular Weight(MW) | 180 kDa |
| Tissue Specificity | This antibody detects endogenous level of IRS-1 only when phosphorylated at serine 639. |
| Entrez Gene | 3667 |
|---|---|
| Protein Accession | NP_005535.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Phospho-Ser639).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using IRS-1 (Phospho-Ser639).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
