Application
Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1 (Phospho-Ser312).| Product Name | IRS 1 (phospho Ser312) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit1) Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1): 63-76Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97 (5): 2355-2360Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645 |
| Immunogen | IRS-1 (Phospho-Ser312) antibody was raised against a peptide sequence around phosphorylation site of serine 312 (A-T-S (p) -P-A) derived from Human IRS-1. |
| Modification | p-Ser312 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRS1 |
|---|---|
| Alternative Names | HIRS-1 OCT1 OTF1 HIRS-1 IRS1 Insulin receptor substrate 1 |
| Molecular Weight(MW) | 180 kDa |
| Tissue Specificity | This antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312. |
| Entrez Gene | 3667 |
|---|---|
| Protein Accession | NP_005535.1 |
Application
Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1 (Phospho-Ser312).| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
