Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ITPA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).Application
Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody at 1:1000 at each lane.| Product Name | ITPA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inosine triphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, ITPase {ECO:0000255|HAMAP-Rule:MF_03148}, Inosine triphosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, 36119 {ECO:0000255|HAMAP-Rule:MF_03148}, Non-canonical purine NTP pyrophosphatase {E |
| Product description | ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.1) Fellay, J., et al. Nature 464(7287):405-408(2010) |
| Immunogen | This ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ITPA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITPA |
|---|---|
| Alternative Names | Inosine triphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148} ITPase {ECO:0000255|HAMAP-Rule:MF_03148} Inosine triphosphatase {ECO:0000255|HAMAP-Rule:MF_03148} 36119 {ECO:0000255|HAMAP-Rule:MF_03148} Non-canonical purine NTP pyrophosphatase {E |
| Molecular Weight(MW) | 21 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ITPA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Application
Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
