Application
Immunohistochemistry staining of JAG1 in kidney tissue using JAG1 Antibody.| Product Name | JAG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JAG1, AGS, AHD, Alagille syndrome, CD339 antigen, HJ1, Jagged1, JAGL1, Jagged, Jagged 1, Protein jagged-1, AWS, CD339, Soluble protein jagged |
| Product description | Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome. A BLAST analysis was used to suggest crossreactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against canine (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. |
| Immunogen | JAG1 antibody was raised against a synthetic peptide corresponding to aa 110 - 125 of JAG1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHCWB |
| JAG1 antibody can be used in Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunocytochemistry.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | JAG1 |
|---|---|
| Alternative Names | JAG1 AGS AHD Alagille syndrome CD339 antigen HJ1 Jagged1 JAGL1 Jagged Jagged 1 Protein jagged-1 AWS CD339 Soluble protein jagged |
Application
Immunohistochemistry staining of JAG1 in kidney tissue using JAG1 Antibody.| Application Notes | JAG1 antibody can be used in Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunocytochemistry.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store JAG1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, pH 7.2, 0.01% sodium azide. |
