Application
Immunohistochemistry of JAG1 in human spleen tissue with JAG1 antibody at 5 μg/ml.Application
Immunofluorescence of JAG1 in human spleen tissue with JAG1 antibody at 20 μg/ml.Application
Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.| Product Name | JAG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1 |
| Product description | The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1). Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1,2). JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3). JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4,5).1) Lai EC. Notch signaling: control of cell communication and cell fate. Development 2004; 131:965-73. |
| Immunogen | JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1. The immunogen is located within the last 50 amino acids of JAG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JAG1 |
|---|---|
| Alternative Names | Jagged 1 AGS AHD AWS CD339 HJ1 JAGL1 |
| Molecular Weight(MW) | Predicted: 134 kDa Observed: 130 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemistry of JAG1 in human spleen tissue with JAG1 antibody at 5 μg/ml.
Application
Immunofluorescence of JAG1 in human spleen tissue with JAG1 antibody at 20 μg/ml.
Application
Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | JAG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | JAG1 antibody is supplied in PBS containing 0.02% sodium azide. |
