Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: HCT116 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | K Cadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cadherin-6, Kidney cadherin, K-cadherin, CDH6 |
| Product description | CDH6 is a type II classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion membrane glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures such as epithelium. Strong transcriptional expression of the CDH6 gene has been observed in hepatocellular and renal carcinoma cell lines, suggesting a possible role in metastasis and invasion.1) Liu,T., J. Proteome Res.(6), 2070-2080 (2005) Shimoyama,Y., Biochem. J. 349 (PT 1), 159-167 (2000) Shimoyama,Y., J. Biol. Chem. 274 (17), 11987-11994 (1999) |
| Immunogen | This K Cadherin (CDH6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-89 amino acids from the N-terminal region of human K Cadherin (CDH6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH6 |
|---|---|
| Alternative Names | Cadherin-6 Kidney cadherin K-cadherin CDH6 |
| Molecular Weight(MW) | 88 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: HCT116 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
