Application
Flow cytometric analysis of NCI-H292 cells using CDH6 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CDH6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-DH6 Antibody in 293 cell line lysates (35ug/lane)| Product Name | K Cadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cadherin-6, Kidney cadherin, K-cadherin, CDH6 |
| Product description | CDH6 is a type II classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion membrane glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures such as epithelium. Strong transcriptional expression of the CDH6 gene has been observed in hepatocellular and renal carcinoma cell lines, suggesting a possible role in metastasis and invasion.1) Liu,T., J. Proteome Res.(6), 2070-2080 (2005) |
| Immunogen | This K Cadherin (CDH6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 665-695 amino acids from the C-terminal region of human K Cadherin (CDH6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH6 |
|---|---|
| Alternative Names | Cadherin-6 Kidney cadherin K-cadherin CDH6 |
| Molecular Weight(MW) | 88 kDa |
Application
Flow cytometric analysis of NCI-H292 cells using CDH6 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CDH6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-DH6 Antibody in 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
