Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
K0090 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse spleen tissue lysates (35ug/lane).Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | K0090 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ER membrane protein complex subunit 1, EMC1, KIAA0090 |
| Product description | 1) Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010) |
| Immunogen | This K0090 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 866-894 amino acids from the C-terminal region of human K0090. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EMC1 |
|---|---|
| Alternative Names | ER membrane protein complex subunit 1 EMC1 KIAA0090 |
| Molecular Weight(MW) | 112 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
K0090 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
