WB
Western blot analysis of EMC1 using anti-EMC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EMC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of EMC1 using anti-EMC1 antibody. EMC1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-EMC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of EMC1 using anti-EMC1 antibody. EMC1 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-EMC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of EMC1 using anti-EMC1 antibody. EMC1 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-EMC1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to KIAA0090/EMC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human KIAA0090/EMC1 recombinant protein (Position: Y23-R993). Human KIAA0090/EMC1 shares 93.7% amino acid (aa) sequence identity with mouse KIAA0090/EMC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-5000 IHC:1:50-400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EMC1 |
|---|---|
| Gene Synonyms | CAVIPMR KIAA0090 |
| Gene Full Name | ER membrane protein complex subunit 1 |
| Gene Summary | This gene encodes a single-pass type I transmembrane protein, which is a subunit of the endoplasmic reticulum membrane protein complex (EMC). Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2012] |
| Alternative Names | EMC1 ER membrane protein complex subunit 1 KIAA0090 |
| Molecular Weight(MW) | 112 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane. |
WB
Western blot analysis of EMC1 using anti-EMC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EMC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of EMC1 using anti-EMC1 antibody. EMC1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-EMC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of EMC1 using anti-EMC1 antibody. EMC1 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-EMC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of EMC1 using anti-EMC1 antibody. EMC1 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-EMC1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:1000-5000 IHC:1:50-400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
