Application
Immunocytochemistry of KPNA1 in HeLa cells with KPNA1 antibody at 2.5 μg/mL.Application
Western blot analysis of KPNA1 in Hela cell lysate with KPNA1 antibody at 1μg/mL.Application
Immunofluorescence of KPNA1 in K562 cells with KPNA1 antibody at 20 μg/mL.| Product Name | KPNA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA1 Antibody: RCH2, SRP1, IPOA5, NPI-1, RCH2, Importin subunit alpha-5, Karyopherin subunit alpha-1 |
| Product description | KPNA1 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA1, also known as importin alpha 5, is the alpha subunit of karyopherin, which forms a complex with importin subunit beta-1 and functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. It is ubiquitously expressed and polyubiquitinated in the presence of RAG1. KPNA1 interacts with various virus nucleoproteins, including those of Ebola and influenza.1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72. |
| Immunogen | KPNA1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA1. The immunogen is located within amino acids 30 - 80 of KPNA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KPNA1 antibody can be used for detection of KPNA1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA1 |
|---|---|
| Alternative Names | KPNA1 Antibody: RCH2 SRP1 IPOA5 NPI-1 RCH2 Importin subunit alpha-5 Karyopherin subunit alpha-1 |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of KPNA1 in HeLa cells with KPNA1 antibody at 2.5 μg/mL.
Application
Western blot analysis of KPNA1 in Hela cell lysate with KPNA1 antibody at 1μg/mL.
Application
Immunofluorescence of KPNA1 in K562 cells with KPNA1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | KPNA1 antibody can be used for detection of KPNA1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KPNA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KPNA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
