WB
Western blot analysis of various lysates using Importin Alpha 5/KPNA1 Rabbit mAb at 1:30000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using Importin Alpha 5/KPNA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using Importin Alpha 5/KPNA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat liver tissue using Importin Alpha 5/KPNA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of PC-3 cells using Importin Alpha 5/KPNA1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with Omnimabs® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab ( dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.IF-P
Confocal imaging of paraffin-embedded Mouse brain using Importin Alpha 5/KPNA1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.IF-P
Confocal imaging of paraffin-embedded Rat spleen using Importin Alpha 5/KPNA1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Product Name | Importin Alpha 5/KPNA1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:30000-1:120000 IHC:1:200-1:2000 ICC/IF:1:200-1:800 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA1 |
|---|---|
| Gene Synonyms | RCH2 SRP1 IPOA5 NPI-1 |
| Gene Full Name | karyopherin subunit alpha 1 |
| Gene Summary | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] |
| Alternative Names | RCH2 SRP1 IPOA5 NPI-1 Importin Alpha 5/KPNA1 |
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates using Importin Alpha 5/KPNA1 Rabbit mAb at 1:30000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using Importin Alpha 5/KPNA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using Importin Alpha 5/KPNA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat liver tissue using Importin Alpha 5/KPNA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of PC-3 cells using Importin Alpha 5/KPNA1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with Omnimabs® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab ( dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
IF-P
Confocal imaging of paraffin-embedded Mouse brain using Importin Alpha 5/KPNA1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
IF-P
Confocal imaging of paraffin-embedded Rat spleen using Importin Alpha 5/KPNA1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:30000-1:120000 IHC:1:200-1:2000 ICC/IF:1:200-1:800 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
