WB
Western blot analysis of Importin Alpha 5/KPNA1 using anti-Importin Alpha 5/KPNA1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Importin Alpha 5/KPNA1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for Importin Alpha 5/KPNA1 at approximately 60 kDa. The expected band size for Importin Alpha 5/KPNA1 is at 60 kDa.FC
Flow Cytometry analysis of U87 cells using anti-Importin Alpha 5/KPNA1 antibody. Overlay histogram showing U87 cells stained with Blue line. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Importin Alpha 5/KPNA1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.ICC/IF
ICC/IF analysis of Importin Alpha 5/KPNA1 using anti-Importin Alpha 5/KPNA1 antibody. Importin Alpha 5/KPNA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Importin Alpha 5/KPNA1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Anti-Importin Alpha 5/KPNA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SRP1/KPNA1, which shares 89.3% and 92.9% amino acid (aa) sequence identity with mouse and rat SRP1/KPNA1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA1 |
|---|---|
| Gene Synonyms | RCH2 SRP1 IPOA5 NPI-1 |
| Gene Full Name | karyopherin subunit alpha 1 |
| Gene Summary | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] |
| Alternative Names | IMA5 Importin alpha 5 Importin subunit alpha 1 Impα5 IPOA5 Karyopherin subunit alpha 1 KPNA1 Nucleoprotein interactor 1 RAG cohort protein 2 RCH2 SRP1 SRP1 beta |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of Importin Alpha 5/KPNA1 using anti-Importin Alpha 5/KPNA1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Importin Alpha 5/KPNA1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for Importin Alpha 5/KPNA1 at approximately 60 kDa. The expected band size for Importin Alpha 5/KPNA1 is at 60 kDa.
FC
Flow Cytometry analysis of U87 cells using anti-Importin Alpha 5/KPNA1 antibody. Overlay histogram showing U87 cells stained with Blue line. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Importin Alpha 5/KPNA1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
ICC/IF
ICC/IF analysis of Importin Alpha 5/KPNA1 using anti-Importin Alpha 5/KPNA1 antibody. Importin Alpha 5/KPNA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Importin Alpha 5/KPNA1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
