Application
Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 μg/mL.Application
Immunocytochemistry of KPNA3 in HeLa cells with KPNA3 antibody at 10 μg/mL.Application
Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | KPNA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA3 Antibody: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma, QIP2, Importin subunit alpha-4, Importin alpha Q2, Qip2 |
| Product description | KPNA3 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA3, the alpha subunit 3 of karyopherin, is silimar to human KPNA2, suggesting that KPNA3 may be involved in the nuclear transport system. It is ubiquitously expressed and recent studies have shown that human KPNA3 may be associated with schizophrenia.1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72. |
| Immunogen | KPNA3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy end of human KPNA3. The immunogen is located within the last 50 amino acids of KPNA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KPNA3 antibody can be used for detection of KPNA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA3 |
|---|---|
| Alternative Names | KPNA3 Antibody: SRP1 SRP4 IPOA4 hSRP1 SRP1gamma QIP2 Importin subunit alpha-4 Importin alpha Q2 Qip2 |
| Sequence Similarities | 3) Cat. No. 17-207 - EL4 Cell Slide |
Application
Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 μg/mL.
Application
Immunocytochemistry of KPNA3 in HeLa cells with KPNA3 antibody at 10 μg/mL.
Application
Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | KPNA3 antibody can be used for detection of KPNA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KPNA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KPNA3 Antibody is supplied in PBS containing 0.02% sodium azide. |
