Application
Antibody used in WB on Human HepG2 cells at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.Application
Antibody used in WB on Human Hela at 1 ug/ml.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.| Product Name | KPNA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA3, IPOA4, SRP1gamma, SRP4, hSRP1, SRP1 |
| Product description | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3 is a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggest that karyopherin alpha-3 may be involved in the nuclear transport system.The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Singh, A.P., (2007) Cell 131 (3), 492-504. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KPNA3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| KPNA3 antibody can be used for detection of KPNA3 by ELISA at 1:12500. KPNA3 antibody can be used for detection of KPNA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA3 |
|---|---|
| Alternative Names | KPNA3 IPOA4 SRP1gamma SRP4 hSRP1 SRP1 |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human HepG2 cells at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.
Application
Antibody used in WB on Human Hela at 1 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | KPNA3 antibody can be used for detection of KPNA3 by ELISA at 1:12500. KPNA3 antibody can be used for detection of KPNA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KPNA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
