Application
Immunocehmistry staining of TPA - induced BCBL - 1 cells using monoclonal antibody 2D4A5 clone to KSHV ORF45. HRP - anti - mouse was used as the second antibody before color development with DAB.Application
Western blot analysis using KSHV ORF45 monoclonal antibody against KSHV ORF45 recombinant protein| Product Name | KSHVORF45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein ORF45 |
| Product description | Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.1) Chang Y. et al. 1994. Science. 266:1865-1869. |
| Immunogen | Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Gene Symbol | ORF45 |
|---|---|
| Alternative Names | Protein ORF45 |
Application
Immunocehmistry staining of TPA - induced BCBL - 1 cells using monoclonal antibody 2D4A5 clone to KSHV ORF45. HRP - anti - mouse was used as the second antibody before color development with DAB.
Application
Western blot analysis using KSHV ORF45 monoclonal antibody against KSHV ORF45 recombinant protein| Application Notes | Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | to KSHV ORF45 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
