| Product Name | LEC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCL16, CKb12, HCC4, ILINCK, LCC1, Liver expressed chemokine, LMC, Monotactin 1, Mtn1, NCC4, SCYA16, SCYL4, Small inducible cytokine A16 precursor, Small inducible cytokine subfamily A (Cys Cys) member 16, C-C motif chemokine 16, Chemokine CC-4, HCC-4, LEC, |
| Product description | LEC displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10. |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hLEC as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL16 |
|---|---|
| Alternative Names | CCL16 CKb12 HCC4 ILINCK LCC1 Liver expressed chemokine LMC Monotactin 1 Mtn1 NCC4 SCYA16 SCYL4 Small inducible cytokine A16 precursor Small inducible cytokine subfamily A (Cys Cys) member 16 C-C motif chemokine 16 Chemokine CC-4 HCC-4 LEC |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | LEC lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
