Application
Western blot analysis of LECT1 using rabbit polyclonal LECT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LECT1 gene.Application
Western blot analysis of anti-LECT1 Antibody in NCI-H460 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | LECT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leukocyte cell-derived chemotaxin 1, Chondrosurfactant protein, CH-SP, Chondromodulin-1, Chondromodulin-I, ChM-I, LECT1, CHMI |
| Product description | LECT1 a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This protein is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development.1) Aoyama,T., Biochem. Biophys. Res. Commun. 365 (1), 124-130 (2008) |
| Immunogen | This LECT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human LECT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LECT1 |
|---|---|
| Alternative Names | Leukocyte cell-derived chemotaxin 1 Chondrosurfactant protein CH-SP Chondromodulin-1 Chondromodulin-I ChM-I LECT1 CHMI |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of LECT1 using rabbit polyclonal LECT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LECT1 gene.
Application
Western blot analysis of anti-LECT1 Antibody in NCI-H460 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
