Application
Western blot analysis of lysed extracts from HeLa and 293 cells using LIN28B Antibody.| Product Name | LIN28B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSDD2, CTNNB, OK/SW-cl.35, PRO2286, CSDD2, FLJ16517 |
| Product description | Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.1) "Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. "Guo Y., Chen Y., Ito H., et al. Gene 384:51-61 (2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. "Heo I., Joo C., Cho J., Ha M., Han J., Kim V. N. Mol. Cell 32:276-284 (2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation. "Heo I., Joo C., Kim Y. -K., Kim V. N., et al. Cell 138:696-708 (2009) |
| Immunogen | LIN28B antibody was raised against a peptide sequence around aa.242~246 ( P-S-V-Q-K) derived from Human LIN28B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIN28B |
|---|---|
| Alternative Names | CSDD2 CTNNB OK/SW-cl.35 PRO2286 CSDD2 FLJ16517 |
| Molecular Weight(MW) | 21 32 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total LIN28B protein. |
| Entrez Gene | 389421 |
|---|---|
| Protein Accession | NP_001004317.1 |
Application
Western blot analysis of lysed extracts from HeLa and 293 cells using LIN28B Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
