Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-LIN28B Pab in K562 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCCIT whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse testis lysate Lane 4: NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | LIN28B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.1) Guo,Y., Gene 384, 51-61 (2006) |
| Immunogen | This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of human LIN28B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIN28B |
|---|---|
| Alternative Names | Protein lin-28 homolog B Lin-28B LIN28B CSDD2 |
| Molecular Weight(MW) | 27 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-LIN28B Pab in K562 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCCIT whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse testis lysate Lane 4: NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
