LPP may play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. LPP may be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. LPP is also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.1) Jin, L., (2007) Circ. Res. 100 (6), 817-825.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LPP.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
LPP antibody can be used for detection of LPP by ELISA at 1:312500. LPP antibody can be used for detection of LPP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human COLO205 at 0.2-1 ug/ml.
Positive Control
1) COLO205 Cell Lysate
Application Notes
LPP antibody can be used for detection of LPP by ELISA at 1:312500. LPP antibody can be used for detection of LPP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store LPP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.