LIM containing lipoma preferred partner, it belongs to the zyxin family. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP, TRIP6 and Zyxin. LPP, an 80 kDa protein and a LIM domain-containing scaffolding protein contains three LIM domains at its carboxy terminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. The LPP localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts, and shuttles to the nucleus where it has transcriptional activation capacity.1) Mol Cell Proteomics. 2005 Sep;4(9):1240-50. Epub 2005 Jun 11.
Immunogen
Purified truncated recombinantLPP-His is expressed in E.Coli strain BL21 (DE3).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
IFIHCWB
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IF:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Western blot analysis using LPP monoclonal antibody against truncated LPP - His recombinant protein and Hela cell lysate.
Positive Control
1) Cat. No. 1201 - HeLa Cell Lysate
Application Notes
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IF:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
LPP monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.