Application
LSP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Western Blot at 1:8000 dilution Lane 1: Daudi whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | LSP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lymphocyte-specific protein 1, 47 kDa actin-binding protein, 52 kDa phosphoprotein, pp52, Lymphocyte-specific antigen WP34, LSP1, WP34 |
| Product description | LSP1 is an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Davila, S., et al. Genes Immun. (2010) |
| Immunogen | This LSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-134 amino acids from the Central region of human LSP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LSP1 |
|---|---|
| Alternative Names | Lymphocyte-specific protein 1 47 kDa actin-binding protein 52 kDa phosphoprotein pp52 Lymphocyte-specific antigen WP34 LSP1 WP34 |
| Molecular Weight(MW) | 37 kDa |
Application
LSP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Western Blot at 1:8000 dilution Lane 1: Daudi whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
